Molecular Formula | C25H25ClN6O |
Molar Mass | 460.97 |
Density | 1.5099 (rough estimate) |
Melting Point | 314 °C |
Water Solubility | SOLUBLE |
Solubility | H2O: 10mg/mL |
Appearance | powder |
Color | Yellow to Very Dark Yellow |
BRN | 4088183 |
Storage Condition | -20°C |
Refractive Index | 1.6300 (estimate) |
MDL | MFCD00012679 |
Use | Commonly used reagents for cytogenetic research, fluorescence staining of cell DNA, insertion into the A- T region of DNA, have low cytotoxicity and form membrane-permeable fluorescent DNA chains. |
In vitro study | Hoechst 33258, a fluorescent compound with a head-to-tail bis-benzimidazole structure, is initially found to be cytotoxic against L1210 murine leukemia. Hoechst 33258 is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), Human promyelocytic leukemia cell (HL60) and U937 cell Line. The IC 50 determined in the case of HeLa, HL60 and U937 is 51.31±4.56, 32.43±3.27 and 15.42±2.16 μM for Hoechst 33258, respectively. The cytotoxic property of Hoechst 33258 is investigated on a panel of seven tumour cell lines of different histological origin and Madine-Darby canine kidney (MDCK) normal cells. All cell lines, except MCF-7, exposed to Hoechst 33258 exhibit GI 50 from 84×10 -6 to 191.5×10 -6 mol/dm 3 . Under the same experimental conditions, Hoechst 33258, used as a binder reference compound, stops the cell cycle in S phase and G0/G1. |
Hazard Symbols | Xn - Harmful |
Risk Codes | R22 - Harmful if swallowed R36/38 - Irritating to eyes and skin. R36/37/38 - Irritating to eyes, respiratory system and skin. R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S24/25 - Avoid contact with skin and eyes. S23 - Do not breathe vapour. |
WGK Germany | 3 |
RTECS | SM1140500 |
FLUKA BRAND F CODES | 3-8-10 |
HS Code | 32129000 |
Reference Show more | 1. Guo bi-ru, Min Yao, Dong Yan, Yuan orange, Qian Jia-Yi, Han Cuiyan. Preparation of erythrocyte membrane coated cucurbitacin B nanostructured lipid carrier and evaluation of its anti-breast cancer effect in vitro [J]. International Journal of Pharmaceutical Research, 2020,47(11):989-994 1000. 2. [IF = 6.986] Xu Jie et al."Red-Near-infra-oral Probe for Time-Resolved in Vivo." Anal Chem. 2019;91(20):13174-13182 |
EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
biological activity | Hoechst 33258 trihydrochloride (bisBenzimide H 33258 trihydrochloride) is a fluorescent dye used for DNA staining. |
target | IC50: 51.31±4.56 μM (HeLa cell), 32.43±3.27 μM (HL60 cell), 15.42±2.16 μM (U937 cell) |
Uses | Commonly used reagents for cytogenetic research, fluorescence staining of cell DNA, insertion into the A- T region of DNA, low cytotoxicity, formation of membrane-permeable fluorescent DNA strand. |